BO HUANG, PhD, Pharmaceutical Chemistry and Biochemistry/Biophysics, UCSF
Description:
ABSTRACT: Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principles and their functional relevance, we are developing new fluorescent labeling methods and microscopy techniques to systematically map the spatial localization, temporal dynamics and activity profiles of proteins. In particular, we have developed the split fluorescent protein tagging method that allows large-scale generation of cell lines with endogenously-labeled proteins by CRISPR/Cas9-mediated gene editing. Correspondingly, we have also built a single-objective high-resolution light-sheet microscope that enables high-throughput imaging of these cell lines. These tools have led to out elucidation of how cytoplasmic protein granules formed by oncogenic kinase fusions activate Ras signaling in cancer cells.
