The Kossiakoff Group's research interests are to provide a molecular understanding of how molecular recognition governs virtually all aspects of biological function. To study these issues our group employs a combination of X-ray crystallography and cryo-EM, site-directed mutagenesis, phage display and biophysical analysis. The Kossiakoff group has also pioneered a new technology called “chaperone-assisted” crystallography, which has facilitated the structural analyses of protein systems that had been totally recalcitrant to other approaches. The group has also been at the forefront of developing synthetic antibodies. These synthetic antibodies are much more powerful that traditional monoclonal antibodies and have the potential to completely replace them for uses in live cell imaging and proteomics.
California Institute of Technology
Postdoc - Structural Biology
1975
University of Delaware
Ph.D - Physical Chemistry
1972
Davis and Elkins College
BS - Chemistry/Math
1968
Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes.
Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes. Protein Sci. 2023 Nov 09; e4824.
PMID: 37945533
Structural and molecular basis of choline uptake into the brain by FLVCR2.
Structural and molecular basis of choline uptake into the brain by FLVCR2. bioRxiv. 2023 Oct 05.
PMID: 37873173
Structure of human drug transporters OATP1B1 and OATP1B3.
Structure of human drug transporters OATP1B1 and OATP1B3. Nat Commun. 2023 09 18; 14(1):5774.
PMID: 37723174
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake.
Chaperone-assisted cryo-EM structure of P. aeruginosa PhuR reveals molecular basis for heme uptake. bioRxiv. 2023 Aug 01.
PMID: 37577460
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry.
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nat Commun. 2023 07 20; 14(1):4368.
PMID: 37474505
ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding.
ACKR3-arrestin2/3 complexes reveal molecular consequences of GRK-dependent barcoding. bioRxiv. 2023 Jul 19.
PMID: 37502840
Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA.
Conformation-specific Synthetic Antibodies Discriminate Multiple Functional States of the Ion Channel CorA. J Mol Biol. 2023 09 01; 435(17):168192.
PMID: 37394032
Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin.
Cryo-EM structures of a synthetic antibody against 22 kDa claudin-4 reveal its complex with Clostridium perfringens enterotoxin. bioRxiv. 2023 Jun 12.
PMID: 37398044
Conformation-specific synthetic antibodies discriminate multiple functional states of the ion channel CorA.
Conformation-specific synthetic antibodies discriminate multiple functional states of the ion channel CorA. bioRxiv. 2023 May 07.
PMID: 37205530
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum.
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum. Structure. 2023 05 04; 31(5):595-606.e5.
PMID: 36977410
Christian Anfinsen Award
Protein Society
2019
Fellow of American Association for Advancement of Science
AAAS
2012
Honorary Degree Doctor of Science
Davis and Elkins College
2011
Alumni Hall of Fame
University of Delaware
2003
Chair Department of Biochemistry and Molecular Biology
University of Chicago
1998 - 2011
Director Institute for Biophysical Dynamics
University of Chicago
1998 - 2003
Director Department of Protein Engineering
Genentech, Inc.
1983 - 1998