Our Faculty

David D Wu, MD PhD

Our lab is interested in how mechanotransduction affects endothelial cell phenotype. We recently discovered that oscillatory shear stress affects endothelial cell metabolism by turning on HIF-1alpha which turns up glycolytic pathways and suppresses mitochondrial function. This metabolic switch, induced by mechanical forces, is necessary for promoting endothelial inflammation. Currently, we are using novel FRET probes to investigate how modulating the actin cytoskeleton affects metabolism at the single cell level.

University of Chicago
- Pulmonary and Critical Care Fellowship
2018

University of Chicago
- Postdoctoral Fellowship, Mutlu and Fang labs
2018

University of Chicago
- Internal Medicine Residency
2014

University of California, Los Angeles
MD - Medicine
2012

California Institute of Technology
PhD - Bioengineering
2010

University of Chicago
BS - Biological Chemistry
2001

Genetic variant at coronary artery disease and ischemic stroke locus 1p32.2 regulates endothelial responses to hemodynamics.
Krause MD, Huang RT, Wu D, Shentu TP, Harrison DL, Whalen MB, Stolze LK, Di Rienzo A, Moskowitz IP, Civelek M, Romanoski CE, Fang Y. Genetic variant at coronary artery disease and ischemic stroke locus 1p32.2 regulates endothelial responses to hemodynamics. Proc Natl Acad Sci U S A. 2018 11 27; 115(48):E11349-E11358.
PMID: 30429326

Role of Cellular Metabolism in Pulmonary Diseases.
Wu D, Woods PS, Duong HT, Mutlu GM. Role of Cellular Metabolism in Pulmonary Diseases. Am J Respir Cell Mol Biol. 2018 07; 59(1):127-129.
PMID: 29634283

Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis.
Hamanaka RB, Nigdelioglu R, Meliton AY, Tian Y, Witt LJ, O'Leary E, Sun KA, Woods PS, Wu D, Ansbro B, Ard S, Rohde JM, Dulin NO, Guzy RD, Mutlu GM. Inhibition of Phosphoglycerate Dehydrogenase Attenuates Bleomycin-induced Pulmonary Fibrosis. Am J Respir Cell Mol Biol. 2018 05; 58(5):585-593.
PMID: 29019702

Letter by Wu et al Regarding Article, "Mechanical Activation of Hypoxia-Inducible Factor 1a Drives Endothelial Dysfunction at Atheroprone Sites".
Wu D, Hamanaka RB, Fang Y, Mutlu GM. Letter by Wu et al Regarding Article, "Mechanical Activation of Hypoxia-Inducible Factor 1a Drives Endothelial Dysfunction at Atheroprone Sites". Arterioscler Thromb Vasc Biol. 2017 12; 37(12):e197-e198.
PMID: 29162600

HIF-1a is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium.
Wu D, Huang RT, Hamanaka RB, Krause M, Oh MJ, Kuo CH, Nigdelioglu R, Meliton AY, Witt L, Dai G, Civelek M, Prabhakar NR, Fang Y, Mutlu GM. HIF-1a is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. Elife. 2017 05 30; 6.
PMID: 28556776

Experimental Lung Injury Reduces Krüppel-like Factor 2 to Increase Endothelial Permeability via Regulation of RAPGEF3-Rac1 Signaling.
Huang RT, Wu D, Meliton A, Oh MJ, Krause M, Lloyd JA, Nigdelioglu R, Hamanaka RB, Jain MK, Birukova A, Kress JP, Birukov KG, Mutlu GM, Fang Y. Experimental Lung Injury Reduces Krüppel-like Factor 2 to Increase Endothelial Permeability via Regulation of RAPGEF3-Rac1 Signaling. Am J Respir Crit Care Med. 2017 03 01; 195(5):639-651.
PMID: 27855271

Transforming Growth Factor (TGF)-ß Promotes de Novo Serine Synthesis for Collagen Production.
Nigdelioglu R, Hamanaka RB, Meliton AY, O'Leary E, Witt LJ, Cho T, Sun K, Bonham C, Wu D, Woods PS, Husain AN, Wolfgeher D, Dulin NO, Chandel NS, Mutlu GM. Transforming Growth Factor (TGF)-ß Promotes de Novo Serine Synthesis for Collagen Production. J Biol Chem. 2016 12 30; 291(53):27239-27251.
PMID: 27836973

Pulse propagation by a capacitive mechanism drives embryonic blood flow.
Anton H, Harlepp S, Ramspacher C, Wu D, Monduc F, Bhat S, Liebling M, Paoletti C, Charvin G, Freund JB, Vermot J. Pulse propagation by a capacitive mechanism drives embryonic blood flow. Development. 2013 Nov; 140(21):4426-34.
PMID: 24089470

A single-molecule Hershey-Chase experiment.
Van Valen D, Wu D, Chen YJ, Tuson H, Wiggins P, Phillips R. A single-molecule Hershey-Chase experiment. Curr Biol. 2012 Jul 24; 22(14):1339-43.
PMID: 22727695

Dynein achieves processive motion using both stochastic and coordinated stepping.
Qiu W, Derr ND, Goodman BS, Villa E, Wu D, Shih W, Reck-Peterson SL. Dynein achieves processive motion using both stochastic and coordinated stepping. Nat Struct Mol Biol. 2012 Jan 08; 19(2):193-200.
PMID: 22231401

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